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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has become observed in lots of tumors, which leads to chromosome instability, and therefore may contribute to tumorigenesis. This protein was observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant position in selling cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been described. Two supplemental splice variants were documented but in-depth nucleotide sequence info is not really yet out there.

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Blocking peptides are peptides that bind particularly to the goal antibody and block antibody binding. These peptide usually includes the epitope recognized from the antibody.

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This manual outlines even more possible optimization of cellular and immunoassay parameters to ensure the very best benefits are...

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This information outlines even further possible optimization of cellular and immunoassay parameters to be sure the very best success are...

Antibodies bound to the blocking peptide no longer bind into the epitope around the focus on protein. By comparing the staining from your blocked antibody as opposed to the antibody by yourself, you can see which staining is certain.

Focus on Facts Cyclin E belongs into the really conserved cyclin family, whose members are characterized by a spectacular periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E varieties a posh with and functions being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S changeover. This protein accumulates with the G1-S section boundary and is particularly degraded as cells development via S period.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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